Lysosomal delivery of ANP receptors following internalization in PC12 cell

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(93)90123-K· OSTI ID:6057919

Rathinavelu, A; Isom, G E ^[1]

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1. Purdue Univ., West Lafayette (India)

Internalization and intracellular processing of ANP-B and C receptors play an important role in regulating cell responsiveness to atrial natriuretic peptides (ANP). Receptor internalization was indirectly monitored with [sup 125]I labelled ligand. When [sup 125]I-ANP(99-126) was internalized by the cells at 37[degrees]C, 55% of the internalized radioactivity was localized in the lysosomal fraction. When receptors were affinity-labelled with [sup 125]I-ANP(99-126) and allowed to internalize for varying time periods, two radiolabelled proteins in the m.wt. range of 56 and 52 KDa were detected in the cytosolic extract. These proteins appear to be the hydrolytic products of the ANP-C receptor expressed on the plasma membrane. In addition to lysosomal delivery, shedding of the ANP-C receptor from the cell surface was detected following incubation of cells with [sup 125]I-ANP(99-126). The dual processes may function to clear exogenous ANP from the extracellular compartments.

• OSTI ID: 6057919
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 53:12; ISSN 0024-3205; ISSN LIFSAK
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HORMONES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LYSOSOMES
MEMBRANE PROTEINS
METABOLISM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
RECEPTORS
TRACER TECHNIQUES