Two classes of single-stranded regions evident in deproteinized preparations of replicating DNA isolated from mammalian cells
- Prince of Wales Children's Hospital, Randwick, Sydney, New South Wales (Australia)
In DNA isolated from proliferating human lymphoblastoid CCRF-CEM cells which had been pulse-labeled by exposure to (3H)thymidine for periods from 30 s to 10 min, single-stranded regions were analyzed by caffeine-gradient elution from benzoylated DEAE-cellulose. Two classes of structural defect were evident. Some replicating DNA exhibited single-stranded regions of approximately 200 nucleotides, while most newly incorporated radioactivity was associated with DNA containing single-stranded regions from 900 to approximately 4000 nucleotides. The distribution of thymidine-derived radioactivity did not suggest sequential or preferential labeling of these DNA fractions as the incorporation time was varied. The findings may be correlated with recent proposals regarding the structural basis of eukaryotic DNA replication.
- OSTI ID:
- 6057280
- Journal Information:
- Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 192:2; ISSN ECREA; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANALEPTICS
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CAFFEINE
CELL PROLIFERATION
CENTRAL NERVOUS SYSTEM AGENTS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DNA
DNA REPLICATION
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PRIMATES
PURINES
PYRIMIDINES
RIBOSIDES
SEPARATION PROCESSES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
XANTHINES