Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: Identification of clones associated with a disease locus

Journal Article · Mon Jul 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.14.6157· OSTI ID:6055943

Nelson, D L; Ballabio, A; Victoria, M F; Pieretti, M; Bies, R D; Gibbs, R A; Maley, J A; Chinault, A C; Webster, T D; Caskey, C T ^[1]

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1. Baylor College of Medicine, Houston, TX (United States)

Over 400 yeast artificial chromosome (YAC) clones were isolated from the human X chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints. Polymerase chain reaction using Alu primers was applied to YAC clones in order to generate probes, to identify overlapping clones, and to derive fingerprints and sequence data directly from total yeast DNA. Several clones were identified in regions of medical interest. One set of three overlapping clones was found to cross a chromsomal translocation implicated in Lowe syndrome. The regional assignment of groups of YAC clones provides initiation points for further attempts to develop large cloned contiguous sequences, as well as material for investigation of regions involved in genetic diseases.

• DOE Contract Number: FG05-88ER60692
• OSTI ID: 6055943
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:14; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
DNA POLYMERASES
DNA-CLONING
ENZYMES
EUMYCOTA
FUNGI
GENETIC MAPPING
HEREDITARY DISEASES
HETEROCHROMOSOMES
HUMAN X CHROMOSOME
HYBRIDIZATION
MAPPING
MICROORGANISMS
MUTATIONS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
PROTEINS
TRANSFERASES
TRANSLOCATION
X CHROMOSOME
YEASTS