Isolation of the functional human excision repair gene ERCC5 by intercosmid recombination
Journal Article
·
· Genomics; (USA)
- Los Alamos National Laboratory, NM (USA)
The complete human nucleotide exicision repair gene ERCC5 was isolated as a functional gene on overlapping cosmids. ERCC5 corrects the excision repair deficiency of Chinese hamster ovary cell line UV135, of complementation group 5. Cosmids that contained human sequences were obtained from a UV-resistant cell line derived from UV135 cells transformed with human genomic DNA. Individually, none of the cosmids complemented the UV135 repair defect; cosmid groups were formed to represent putative human genomic regions, and specific pairs of cosmids that effectively transformed UV135 cells to UV resistance were identified. Analysis of transformants derived from the active cosmid pairs showed that the functional 32-kbp ERCC5 gene was reconstructed by homologous intercosmid recombination. The cloned human sequences exhibited 100% concordance with the locus designated genetically as ERCC5 located on human chromosome 13q. Cosmid-transformed UV135 host cells repaired cytotoxic damage to levels about 70% of normal and repaired UV-irradiated shuttle vector DNA to levels about 82% of normal.
- OSTI ID:
- 6053891
- Journal Information:
- Genomics; (USA), Journal Name: Genomics; (USA) Vol. 8:4; ISSN GNMCE; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells
Molecular cloning and characterization of a mammalian excision repair gene that partially restores UV resistance to xeroderma pigmentosum complementation group D cells
Human ERCC5 cDNA-cosmid complementation for excision repair and bipartite amino acid domains conserved with RAD proteins of saccharomyces cerevisiae and schizosaccharomyces pombe
Journal Article
·
Mon Feb 29 23:00:00 EST 1988
· Mol. Cell. Biol.; (United States)
·
OSTI ID:5131564
Molecular cloning and characterization of a mammalian excision repair gene that partially restores UV resistance to xeroderma pigmentosum complementation group D cells
Journal Article
·
Fri Sep 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5407022
Human ERCC5 cDNA-cosmid complementation for excision repair and bipartite amino acid domains conserved with RAD proteins of saccharomyces cerevisiae and schizosaccharomyces pombe
Journal Article
·
Fri Oct 01 00:00:00 EDT 1993
· Molecular and Cellular Biology; (United States)
·
OSTI ID:5852645
Related Subjects
550400 -- Genetics
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMES
DNA REPAIR
ELECTROMAGNETIC RADIATION
EXCISION REPAIR
GENE RECOMBINATION
GENES
GENETIC MAPPING
MAMMALS
MAN
MAPPING
NUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VERTEBRATES
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMES
DNA REPAIR
ELECTROMAGNETIC RADIATION
EXCISION REPAIR
GENE RECOMBINATION
GENES
GENETIC MAPPING
MAMMALS
MAN
MAPPING
NUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VERTEBRATES