Enumeration of 6-thioguanine-resistant T-lymphocytes in the peripheral blood of nonhuman primates (cynomolgus monkeys)

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850180304· OSTI ID:6052473

Zimmer, D M; Aaron, C S ^[1]; O'Neill, J P; Albertini, R J ^[2]

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1. UpJohn Company, Kalamazoo, MI (United States)
2. Univ. of Vermont, Burlington (United States)

The authors have investigated the use of cynomolgus monkeys (Macaca fascicularis) as a model of somatic cell mutagenesis in non-human primates. Using techniques described by Albertini for similar studies in humans, the frequency of TG-resistant T-lymphocytes in the peripheral blood was determined in animals that were either untreated or treated with ethylnitrosourea. Maximum frequency of TG-resistant cells for two animals treated with a single I.P. dose of ENU was 45.1 and 77.9 per 10{sup 6} cells. Substantial increases in frequencies of TG-resistant cells were not seen until at least 63 days after treatment. The TG-resistant phenotype of clones isolated in the assay was stable after growth for 2 weeks in the absence of selective agent.

• OSTI ID: 6052473
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:3; ISSN EMMUE; ISSN 0893-6692
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
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CONNECTIVE TISSUE CELLS
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MUTAGENESIS
MUTATION FREQUENCY
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ORGANIC NITROGEN COMPOUNDS
PRIMATES
SOMATIC CELLS
SOMATIC MUTATIONS
VERTEBRATES