Genetic study on the effects of the repair deficient mutants mei-9/sup a/, mei-41/sup D5/, mus101/sup D1/, mus104/sup D1/, and mus302/sup D1/ of Drosophila melanogaster on x-ray induced chromosome loss and translocations in the paternal genome

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1981

OSTI ID:6047779

Cooper, S F

Each mutant was incorporated singly into ring-x males each provided with V/sup s/Yy/sup +/. From matings of males carrying mus101/sup D1/, mus302/sup D1/, or mei-41/sup D5/, mutants identifying a caffeine sensitive (CAS) postreplication repair pathway, with corresponding mutant females, overall frequencies of spontaneous partial loss and spontaneous complete loss were significantly increased over nonmutant controls. Similar findings were noted when males carrying the excision repair mutant mei-9/sup a/ were mated with mei-9/sup a/ females. Males carrying the mutant mus104/sup D1/, identifying a caffeine insensitive (CIS) postreplication repair pathway, tested with mus104/sup D1/ females, produced results that were not significantly different from non-mutant controls. When males were given 3000 rads x-irradiation, frequencies of induced partial loss were significantly higher with mus101/sup D1/, mus302/sup D1/, mei-41/sup D5/, and mei-9/sup a/ and not significantly different from controls with mus104/sup D1/. It was suggested that the functional CAS postreplication repair pathway primarily promotes repair, while an alternative pathway(s), not defined by mus104/sup D1/, promotes misrepair. Therefore, the significant increases in both spontaneous and induced partial loss with the excision repair deficient mutant mei-9/sup a/ suggest the possibility that (a) the excision repair pathway may not function in misrepair and (b) the undefined misrepair pathway may be the dominant pathway for postreplication repair in Drosophila since mei-9/sup a/ females presumably have functional repair and misrepair capacity.

• Research Organization: Brown Univ., Providence, RI (USA)
• OSTI ID: 6047779
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMOSOMAL ABERRATIONS
BIOLOGICAL REPAIR
DROSOPHILA
GENETIC RADIATION EFFECTS
BIOLOGICAL PATHWAYS
COMPARATIVE EVALUATIONS
GENES
MUTANTS
TRANSLOCATION
X RADIATION
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
DIPTERA
ELECTROMAGNETIC RADIATION
FLIES
FRUIT FLIES
GENETIC EFFECTS
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
560153* - Radiation Effects on Animals- Invertebrates- (-1987)