Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant

Journal Article · Thu Feb 14 23:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.4.1187· OSTI ID:6045637

Bliska, J B; Falkow, S ^[1]; Guan, Kunliang; Dixon, J E ^[2]

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1. Stanford Univ., CA (United States)
2. Purdue Univ., West Lafayette, IN (United States)

The plasmid-encoded YopH protein is a protein-tyrosine phosphatase that is essential for Yersinia virulence. The authors have investigated the molecular basis for the role of PTPase activity in Yersinia pathogenesis. Allelic recombination was employed to introduce a defined mutation in to the yopH plasmid gene. Conversion of the essential Cys-403 to Ala in the catalytic domain of the protein abolished YopH PTPase activity and significantly reduced the virulence of Yersinia pseudotuberculosis in a murine infection model. {sup 32}P-labeled phosphotyrosine-containing proteins were immunoprecipitated from extracts of Y. pseudotuberculosis-infected cell monolayers and analyzed by SDS/PAGE to assess the impact of YopH on host protein phosphorylation. Major proteins of 200, 120, and 60 kDa were dephosphorylated in macrophages associated with wild-type Y.pseydotuberculosis. Selective removal of phosphate from the 120- and 60-kDa proteins was shown to be specific to the YopH PTPase activity. These observations indicate that the essential function of YopH in Yersinia pathogenesis is host-protein dephosphorylation.

• OSTI ID: 6045637
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:4; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOTECHNOLOGY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DISEASES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
ESTERASES
GENE RECOMBINATION
GENETIC ENGINEERING
HYDROLASES
HYDROLYSIS
HYDROXY ACIDS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LYSIS
MACROPHAGES
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
PLASMIDS
PROTEINS
RADIOISOTOPES
SOLVOLYSIS
SOMATIC CELLS
TYROSINE