Direct evidence that ganglioside is an integral component of the thyrotropin receptor

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.5.1991· OSTI ID:6045233

Kielczynski, W; Harrison, L C; Leedman, P J ^[1]

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1. Walter and Eliza Hall Inst. of Medical Research, Melbourne, Victoria (Australia)

Gangliosides were extracted from purified human and porcine thyrotropin (TSH) receptors (TSH-R) and were detected by probing with an {sup 125}I-labeled sialic acid-specific lectin, Limax flavus agglutinin. Gangliosides copurified with human and porcine TSH-R migrated between monosialoganglioside GM1 and disialoganglioside GD1a. Ceramide glycanase digestion of the purified human TSH-R-associated glycolipid confirmed its ganglioside nature. It was resistant to Vibrio cholerae sialidase, which digest all gangliosides except GM1, but was sensitive to Arthrobacter ureafaciens sialidase, which digests all gangliosides including GM1. These findings indicate that the human TSH-R contains ganglioside that belongs to the galactosyl({beta}1{r arrow} 3)-N-acetylgalactosaminyl({beta}1{r arrow} 4)-(N-acetylneuraminyl({alpha}2{r arrow} 3))galactosyl({beta}1 {r arrow} 4)glucosyl({beta}1 {r arrow} 1)ceramide (GM1) family. Its intimate association with receptor protein implies a key role for ganglioside in the structure and function of the TSH-R.

• OSTI ID: 6045233
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:5; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GANGLIOSIDES
BIOCHEMISTRY
RECEPTORS
MOLECULAR STRUCTURE
TSH
RADIORECEPTOR ASSAY
ELECTROPHORESIS
GLYCOPROTEINS
IODINE 125
MAN
SIALIC ACID
SWINE
ANIMALS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOLIPIDS
HORMONES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
MAMMALS
MEMBRANE PROTEINS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques