Affinity labeling the bovine gallbladder cholecystokinin receptor using a battery of probes

Journal Article · Tue Nov 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6038825

Schjoldager, B; Powers, S P; Miller, L J ^[1]

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1. Mayo Clinic and Foundation, Rochester, MN (USA)

Although the gallbladder was the first recognized target of the peptide hormone cholecystokinin (CCK) and is a physiologically important target, only one preliminary report of the biochemical characterization of this receptor exists. Recently, a series of molecular probes for the affinity labeling of different domains of the pancreatic CCK receptor have been developed. In this work the authors report the application of several of those probes toward the biochemical characterization of the bovine gallbladder muscularis receptor. These include long ({sup 125}I-Bolton-Hunter-CCK-33) and short ({sup 125}I-D-Tyr-Gly-((Nle{sup 28,31})CCK-(26-33))) probes chemically cross-linkable through their amino-terminal amino groups and monofunctional probes with their photolabile moieties at their amino terminus (2-diazo-3,3,3-trifluoropropionyl-{sup 125}I-D-Tyr-Gly-((Nle{sup 28,31})CCK-(26-33))) and carboxyl terminus ({sup 125}I-D-Tyr-Gly-((Nle{sup 28,31},pNO{sub 2}-Phe{sup 33})CCK-(26-33))), that span the receptor-binding region. Each of these bound specifically and saturably to a preparation enriched in plasma membranes from bovine gallbladder muscularis. These observations support the identification of the M{sub r} 70,000-85,000 protein as the bovine gallbladder CCK-binding subunit and, since this is a different size from the pancreatic CCK-binding subunit, provide biochemical evidence for molecular heterogeneity of peripheral CCK receptors.

• OSTI ID: 6038825
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CATTLE
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KININS
LABELLING
MAMMALS
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RECEPTORS
RUMINANTS
VERTEBRATES