Myocardial neutrophil accumulation during reperfusion after reversible or ischemic injury

Journal Article · Tue Nov 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6037028

Go, L O; Murry, C E; Richard, V J; Weischedel, G R; Jennings, R B; Reimer, K A ^[1]

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1. Duke Univ. Medical Center, Durham, NC (USA)

Recent studies suggest that polymorphonuclear leukocytes (PMNs) may cause additional myocyte injury during reperfusion of ischemic myocardium. The present study was done to investigate whether PMNs accumulate in myocardium during early reperfusion after reversible or irreversible ischemic injury. Open-chest anesthetized dogs underwent circumflex coronary occlusions for 12 min, 40 min, or 90 min, followed by 1 h of reperfusion. Autologous PMNs were radiolabeled with {sup 111}In and reinjected to quantitate myocardial PMN influx during reflow. {sup 125}I-labeled albumin was injected simultaneously to correct for {sup 111}In associated with plasma proteins in myocardial tissue. The number of PMNs was determined in the inner, middle, and outer one-third of nonischemic and ischemic-reperfused myocardium. In the 12-min group, 40% fewer PMNs were present in the reperfused than in the nonischemic control tissue. In contrast, in both the 40- and 90-min groups, PMN accumulation was two- to six-fold greater in the ischemic-reperfused than nonischemic myocardium, with a transmural gradient of PMN influx increasing from the outer to inner layers. Collateral blood flow, measured with radioactive microspheres, was not significantly different among the three groups. The failure of PMNs to accumulate during reperfusion after 12 min of ischemia does not support the hypothesis that PMNs contribute to postischemic dysfunction of reversibly injured myocytes. Whether PMNs cause cell death during early reperfusion after longer ischemic episodes remains unknown; however, the rapidity of PMN accumulation in the zones of predicted infarction is consistent with this possibility.

• OSTI ID: 6037028
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
HEART
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISCHEMIA
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
MYOCARDIUM
NECROSIS
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PERFUSED TISSUES
RADIOISOTOPES
TISSUES
UPTAKE
VASCULAR DISEASES
VERTEBRATES