Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII
The nucleotide sequence of the region of the Epstein-Barr virus genome that specified two small ribonucleic acids (RNAs), EBER 1 and EBER 2, has been determined. Both of these RNAs are encoded by the right-hand 1,000 base pairs of the EcoRI J fragment of EBV deoxyribonucleic acid. EBER 1 is 166 (167) nucleotides long and EBER 2 is 172 +- 1 nucleotides long; the heterogeneity resides at the 3' termini. The EBER genes are separated by 161 base pairs and are transcribed from the same deoxyribonucleic acid strand. In vitro, both EBER genes can be transcribed by RNA polymerase III; sequences homologous to previously identified RNA polymerase III intragenic transcription control regions are present. Striking similarities are therefore apparent both between the EBERs and the two adenovirus-associated RNAs, VAI and VAII, and between the regions of the two viral genomes that specify these small RNAs. We have shown that VAII RNA as well as VAI RNA and the EBERs exist in ribonucleoprotein complexes which are precipitable by anti-La antibodies associated with systemic lupus erythematosus. Finally the authors have demonstrated that the binding of protein(s) from uninfected cells confers antigenicity on each of the four virus-encoded small RNAs.
- Research Organization:
- Dept. of Molecular Biophysics and Biochemistry, Yale Univ., New Haven, CT 06510
- OSTI ID:
- 6030441
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 1:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADENOVIRUS
BIOLOGICAL FUNCTIONS
INFECTIVITY
CONNECTIVE TISSUE
ANTIGEN-ANTIBODY REACTIONS
DISEASES
DNA POLYMERASES
NUCLEOPROTEINS
GENES
GENETIC MAPPING
SPECIES DIVERSITY
RNA
DNA SEQUENCING
RNA POLYMERASES
ANTIBODIES
GENETIC VARIABILITY
IMMUNE REACTIONS
PRECIPITATION
TRANSCRIPTION
ANIMAL TISSUES
BIOLOGICAL VARIABILITY
BODY
ENZYMES
FUNCTIONS
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
TRANSFERASES
VIRUSES
550200* - Biochemistry
550400 - Genetics