Protein biosynthesis with conformationally restricted amino acids
- Univ. of California, Berkeley (United States) Lawrence Berkeley Lab., CA (United States)
- Univ. of California, Berkeley (United States)
The incorporation of conformationally constrained amino acids into peptides is a powerful approach for generating structurally defined peptides as conformational probes and bioactive agents. The ability to site-specifically introduce constrained amino acids into large polypeptide chains would provide a similar opportunity to probe the flexibility, conformation, folding and stability of proteins. To this end, we have examined the competence of the Escherichia coli protein biosynthetic machinery to incorporate a number of these unnatural amino acids into the 164 residue protein T4 lysozyme (T4L). Results clearly demonstrate that the protein biosynthetic machinery can accommodate a wide variety of conformationally constrained amino acids. The expansion of structural motifs that can be biosynthetically incorporated into proteins to include a large number of conformationally constrained amino acids significantly increases the power of mutagenesis methods as probes of protein structure and function and provides additional insights into the steric requirements of the translational machinery. 13 refs., 2 figs.
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6030350
- Journal Information:
- Journal of the American Chemical Society; (United States), Journal Name: Journal of the American Chemical Society; (United States) Vol. 115:10; ISSN JACSAT; ISSN 0002-7863
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400201 -- Chemical & Physicochemical Properties
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BIOSYNTHESIS
CARBOXYLIC ACIDS
CONFORMATIONAL CHANGES
ENZYMES
ESCHERICHIA COLI
GLYCOSYL HYDROLASES
HYDROLASES
LYSOZYME
MICROORGANISMS
MOLECULAR STRUCTURE
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
SYNTHESIS