Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance

Journal Article · Wed Dec 31 04:00:00 EST 1997 · FEMS Microbiology Letters

DOI:https://doi.org/10.1016/S0378-1097(97)00028-1· OSTI ID:603034

Mai, V; Lorenz, W W; Wiegel, J ^[1]

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1. Univ. of Georgia, Athens, GA (United States)

The industrial application of thermophilic (eu)bacteria is hampered by the lack of genetic systems for these bacteria. We report here the first unequivocal transformation of a Gram-positive, thermophilic, anaerobic microorganism, Thermoanaerobacterium, with the kanamycin resistance-mediating plasmid pIKM1. The construct pIKM1 is based on the Escherichia coli-Clostridium acetobutylicum shuttle vector pIMP1 and contains the thermostable kanamycin cassette from S. faecalis plasmid pKD102. Using electrotransformation, plasmid pIKM1 mediated kanamycin resistance in Thermoanaerobacterium sp. strain JW/SL-YS485 up to 400 {mu}g ml{sup -1} at 48{degrees}C and 200 {mu}g ml{sup -1} at 60{degrees}C.

• DOE Contract Number: FG05-95ER20199
• OSTI ID: 603034
• Journal Information: FEMS Microbiology Letters, Journal Name: FEMS Microbiology Letters Vol. 148; ISSN 0378-1097; ISSN FMLED7
• Country of Publication: Netherlands
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CLOSTRIDIUM ACETOBUTYLICUM
ESCHERICHIA COLI
GENETIC ENGINEERING
PLASMIDS