Modulation of complement component biosynthesis by human lung cells

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6030261

Rothman-Sabia, B L

Research has focused on evaluating the capability of lung tissue cells to biosynthesize complement components in vitro, and the regulation of this production, utilizing cell lines of human type II pneumocytes (A549) and lung fibrblasts (WI-38). Initially complement biosynthesis was directly demonstrated by incorporation of /sup 35/S-methionine into immunoprecipitable complement components and regulatory proteins. Using this procedure, synthesis of Clr, Cls, C4, C3, C5, C6, C7, C8, C9, Factors B, H, I and Cls inactivator by A549 was detected, but not Clq or albumin. Quantitative evaluation of the kinetics of C3 and C5 production demonstrated that A549 produced antigenic C3, antigenic C5 and functional C5. In contrast, the WI-38 produced C3 and C5 at 30 percent the rates determined for A549. The effects of exogenous agents and endogenous factors to modulate complement component production by A549 and WI-38 were evaluated.

• Research Organization: Connecticut Univ., Storrs (USA)
• OSTI ID: 6030261
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COMPLEMENT
BIOSYNTHESIS
FIBROBLASTS
LUNGS
MAN
METHIONINE
RADIOIMMUNOASSAY
SULFUR 35
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RESPIRATORY SYSTEM
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques