Flow cytometry for health monitoring in space

Conference · Sun Jan 01 00:00:00 EST 1984

OSTI ID:6028917

Jett, J H; Martin, J C; Saunders, G C; Stewart, C C

Monitoring the health of space station or lunar base residents will be necessary to provide knowledge of the physiological status of astronauts. Flow cytometric techniques are uniquely capable of providing cellular, chromosome, hormone level and enzyme level information. The use of dyes provides the basis for fluorescently labeling specific cellular components. Laser induced fluorescence from stained cells is quantitated in a flow cytometer to measure cellular components such as DNA, RNA and protein. One major application of a flow cytometer will be to perform a complete blood count including hematocrit, hemoglobin content, and numbers of platelets, erythrocytes, granulocytes, lymphocytes and monocytes. A newly developed flow cytometry based fluoroimmunoassay will be able to measure levels of serum enzymes and hormones. It will also be possible to quantitate radiation exposure and some forms of chromosome damage with flow cytometric measurements. With relatively simple modifications to existing technology, it will be possible to construct a flight rated cytometer. 11 references, 6 figures, 2 tables.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6028917
• Report Number(s): LA-UR-85-802; CONF-8410230-11; ON: DE85009572
• Resource Relation: Conference: Lunar bases and space activities of the 21st century, Washington, DC, USA, 29 Oct 1984
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD CHEMISTRY
BIOASSAY
CELL FLOW SYSTEMS
DIAGNOSTIC USES
SPACE FLIGHT
BLOOD CELLS
BLOOD COUNT
CHROMOSOMAL ABERRATIONS
FLUORESCENCE
LABELLING
PERSONNEL
RADIATION DOSES
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DOSES
LUMINESCENCE
MATERIALS
MUTATIONS
USES
550600* - Medicine