Identification of antigenic proteins of setaria cervi by immunoblotting technique

Journal Article · Tue Mar 31 23:00:00 EST 1987 · Immunolog. Invest.; (United States)

DOI:https://doi.org/10.3109/08820138709030571· OSTI ID:6028244

Kaushal, N A; Kaushal, D C; Ghatak, S

Identification and characterization of antigenic proteins of Setaria cervi (bovine filarial parasite) adults and microfilariae was done by immunoblotting technique using hyperimmune rabbit sera against S. cervi and Brugia malayi. The antigens recognized by these sera were detected by using /sup 125/I protein-A followed by autoradiography. Fifteen different antigens were observed to be common between adult and microfilarial stages of the parasite. Some stage specific antigens were also identified. Many antigens of S. cervi adults and microfilariae were also recognized by rabbit anti-B.malayi serum showing the existence of common antigenic determinants between the bovine and human filarial parasites.

• Research Organization: Central Drug Research Institute, Lucknow, India
• OSTI ID: 6028244
• Journal Information: Immunolog. Invest.; (United States), Journal Name: Immunolog. Invest.; (United States) Vol. 16:2; ISSN IMINE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
CATTLE
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PRIMATES
PROTEINS
RADIOISOTOPES
RUMINANTS
VERTEBRATES