Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.7.2007· OSTI ID:6027580

Medof, M E; Lublin, D M; Holers, V M; Ayers, D J; Getty, R R; Leykam, J F; Atkinson, J P; Tykocinski, M L

cDNAs encoding the complement decay-accelerating factor (DAF) were isolated from HeLa and differentiated HL-60 lambdagt cDNA libraries by screening with a codon preference oligonucleotide corresponding to DAF NH/sub 2/-terminal amino acids 3-14. The composite cDNA sequence showed a 347-amino acid protein preceded by an NH/sub 2/-terminal leader peptide sequence. The translated sequence beginning at the DAF NH/sub 2/ terminus encodes four contiguous approx. = 61-amino acid long repetitive units of internal homology. The repetitive regions contain four conserved cysteines, one proline, one glycine, one glycine/alanine, four leucines/isoleucines/valines, one serine, three tyrosines/phenylalanines, and on tryptophan and show striking homology to similar regions previously identified in factor B, C2, C4 binding protein, factor H, C1r, factor XIII, interleukin 2 receptor, and serum ..beta../sub 2/-glycoprotein I. The consensus repeats are attached to a 70-amino acid long segment rich in serine and threonine (potential O-glycosylation sites), which is in turn followed by a stretch of hydrophobic amino acids. RNA blot analysis of HeLa and HL-60 RNA revealed three DAF mRNA species of 3.1, 2.7, and 2.0 kilobases. The results indicate that portions of the DAF gene may have evolved from a DNA element common to the above proteins, that DAF cDNA predicts a COOH-terminal anchoring polypeptide, and that distinct species of DAF message are elaborated in cells.

• Research Organization: Case Western Reserve Univ., Cleveland, OH
• OSTI ID: 6027580
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:7; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
CLONING
COMPLEMENT
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DNA-CLONING
HELA CELLS
ISOTOPES
LIGHT NUCLEI
MEMBRANES
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS