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Effect of beta-amino-propionitrile on the regenerating newt limb evaluated by uptake of /sup 3/H-proline and /sup 3/H-lysine

Journal Article · · Growth; (United States)
OSTI ID:6027398
Newt forelimbs were unilaterally amputated and Beta-amino propionitrile (BAPN) was added to the water in which the newts were maintained. Sixty-three days after amputation, animals were injected with either /sup 3/H-proline or /sup 3/H-lysine. Forelimbs distal to the elbow of both amputated and non-amputated sides were removed 72 hours later. Uptake of /sup 3/H-proline and /sup 3/H-lysine in whole limbs was assessed by liquid scintillation and in cartilage cells and matrix by autoradiography. Amputation was a potent stimulus to both collagen formation and maturation during regeneration. BAPN reduced the stimulatory effect of amputation on collagen formation but not on its maturation. Collagen synthesis and maturation are independent events and, in intact non-regenerating limbs, BAPN adversely affected collagen maturation while collagen synthesis was unaltered.
Research Organization:
New York College of Osteopathic Medicine, Old Westbury
OSTI ID:
6027398
Journal Information:
Growth; (United States), Journal Name: Growth; (United States) Vol. 51:1; ISSN GROWA
Country of Publication:
United States
Language:
English