Choline incorporation by Schistosoma mansoni: distribution of choline metabolites during development and after sexual differentiation
Choline metabolism was investigated in Schistosoma mansoni during the main phases of its development, namely, schistosomula, 11- and 15-day-old worms, and adults. At the physiological choline concentration used in the assay (20 microM), betaine was, along with phosphatidylcholine, one of the most abundant choline metabolites, revealing considerable choline oxidation activity. Very little radioactivity was associated with CDP-choline, whereas a sustained incorporation into phosphocholine occurred. These results provide good evidence that CTP:phosphocholine cytidylyltransferase plays a regulatory role in the de novo pathway of phosphatidylcholine biosynthesis. During development, the incorporation of choline into its various metabolites was maximal in 11-day-old worms. At this stage, the oxidative pathway predominated over the Kennedy pathway, whereas at all other stages the de novo phosphatidylcholine biosynthesis was predominant. Furthermore, choline incorporation into betaine was much more important in the adult female worm than in the male, indicating a major difference in choline incorporation and distribution between the 2 sexes of the adult worms.
- Research Organization:
- INSERM U.58-CNRS UA 530, Montpellier, France
- OSTI ID:
- 6025411
- Journal Information:
- J. Parasitol.; (United States), Vol. 73:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHOLINE
METABOLISM
SCHISTOSOMA
CELL DIFFERENTIATION
TRANSFERASES
ENZYME ACTIVITY
BETAINE
BIOLOGICAL PATHWAYS
METABOLITES
RADIOISOTOPES
SEX DEPENDENCE
TRACER TECHNIQUES
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
CARBOXYLIC ACIDS
DRUGS
ENZYMES
HELMINTHS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLATYHELMINTHS
QUATERNARY COMPOUNDS
TREMATODES
550501* - Metabolism- Tracer Techniques