Choline or methionine reverses impaired secretion of VLDL by hepatocytes from choline-deficient rats
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6024049
Male rats fed a choline-deficient (CD) diet for three days accumulated triacylglycerol (TG) in the liver. Hepatocytes from these rats were cultured and maintained in a medium + choline. The rate of secretion of TG was reduced by 50% in the CD cells. Correspondingly, (/sup 3/H)oleate and (/sup 3/H)glycerol were incorporated at a 2-fold higher rate into TG secreted by choline-supplemented cells compared to CD cells. Isolation of lipoprotein fractions by ultracentrifugation showed that the reduced secretion of TG by CD hepatocytes was mainly due to an impaired secretion of very low density lipoprotein (VLDL). Incorporation of (/sup 3/H)leucine into secreted apoB/sub H/, apoB/sub L/ and apoE was markedly reduced in CD cells compared to choline-supplemented cells. Secretion of high density lipoprotein was not reduced in the CD hepatocytes. Normal secretion of VLDL was resumed upon addition of methionine to the CD cells.
- Research Organization:
- Univ. of Alberta, Edmonton
- OSTI ID:
- 6024049
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
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Fasting decreases apolipoprotein B mRNA editing and the secretion of small molecular weight apoB by rat hepatocytes: Evidence that the total amount of apoB secreted is regulated post-transcriptionally
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· Journal of Lipid Research; (USA)
·
OSTI ID:6229743
Requirement of cholesterol for secretion of the very low density lipoprotein (VLDL) by the rat liver
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6326544
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CENTRIFUGATION
CHOLINE
DRUGS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUCINE
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
LIVER CELLS
MAMMALS
METHIONINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
QUATERNARY COMPOUNDS
RATS
REACTION KINETICS
RODENTS
SECRETION
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CENTRIFUGATION
CHOLINE
DRUGS
GLYCEROL
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUCINE
LIPIDS
LIPOPROTEINS
LIPOTROPIC FACTORS
LIVER CELLS
MAMMALS
METHIONINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
QUATERNARY COMPOUNDS
RATS
REACTION KINETICS
RODENTS
SECRETION
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES