Environmental carcinogens in human target tissues in culture. Progress report
Cells from different organ or animal species have shown diverse activities in activation and detoxification of chemical carcinogens. Based on the mutation assays, human hepatocytes were more effective than animal hepatocytes in detoxification of aromatic nitrogen compounds. The adduct formation was also different in human and rodent hepatocytes exposed to aminofluorene (AF) or acetylaminofluorene (AAF). Both AF and AAF adduct DNA were observed in rat liver cells exposed to AF or AAF. However, very little acetylation or deacetyl of the DNA adducts occurred in the human hepatocytes. Human hepatocytes treated with AF in primary culture produced mainly AF adducted DNA while AAF treated cells formed AAF adduct DNA. 2 figs., 1 tab.
- Research Organization:
- Maryland Univ., Baltimore (USA). Dept. of Pathology
- DOE Contract Number:
- AS05-83ER60158
- OSTI ID:
- 6024028
- Report Number(s):
- DOE/ER/60158-2; ON: DE86009802
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
ADDUCTS
AFLATOXIN
AMINES
ANIMALS
ANTIGENS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
DATA
DNA ADDUCTS
EXPERIMENTAL DATA
INFORMATION
MALES
MAMMALS
MAN
MATERIALS
MEN
METABOLIC ACTIVATION
MORPHOLOGICAL CHANGES
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC AMINES
PRIMATES
RATS
RODENTS
SISTER CHROMATID EXCHANGES
SPECIES DIVERSITY
TOXIC MATERIALS
TOXINS
ULTRASTRUCTURAL CHANGES
VERTEBRATES