Aspects of sucrose transport in stem parenchyma of sweet sorghum. [Sorghum bicolor]

Conference · Sat Aug 01 04:00:00 EDT 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:6021119

Lingle, S E

Sweet sorghum (Sorghum bicolor (L.) Moench) is a sucrose-storing crop with a storage tissue anatomically similar to that of sugarcane (Saccharum spp.). However, recent evidence suggests that sweet sorghum may be biochemically different from sugarcane. /sup 14/C-sucrose uptake was studied in excised tissue discs from fully-elongated internodes of Rio sweet sorghum. Washout studies gave results consistent with a 3 compartment system. After 3 hours of uptake, most of the /sup 14/C was found in the vacuole compartment, and was determined by HPLC to be sucrose. Total sucrose uptake consisted of a PCMBS-sensitive (active) and a PCMBS-insensitive (passive) component. Active sucrose uptake had a pH optimum of 4.5. Total sucrose uptake was negatively correlated with the internal sucrose content of the tissue. Fructosyl-labelled /sup 14/C-sucrose was not randomized during uptake, suggesting that sucrose cleavage is not a requirement for sucrose uptake in sweet sorghum. This data suggests that in sweet sorghum, sucrose is transported intact by a specific carrier, as opposed to the sucrose-cleavage-and-resynthesis transport system that apparently operates in sugarcane.

• Research Organization: Dept. of Agriculture-ARS, Weslaco, TX
• OSTI ID: 6021119
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CEREALS
CHEMISTRY
CHROMATOGRAPHY
DISACCHARIDES
GRASS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PH VALUE
PHYSIOLOGY
PLANT STEMS
PLANTS
SACCHARIDES
SACCHAROSE
SEPARATION PROCESSES
SORGHUM
TRACER TECHNIQUES
TRANSLOCATION