Replication-defective vectors of reticuloendotheliosis virus transduce exogenous genes into somatic stem cells of the unincubated chicken embryo

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · J. Virol.; (United States)

OSTI ID:6017506

Bosselman, R A; Hsu, R Y; Boggs, T; Hu, S; Bruszewski, J; Ou, S; Souza, L; Kozar, L; Martin, F; Nicolson, M

Replication-defective vectors derived from reticuloendotheliosis virus were used to transduce exogenous genes into early somatic stem cells of the chicken embryo. One of these vectors transduced and expressed the chicken growth hormone coding sequence. The helper cell line, C3, was used to generate stocks of vector containing about 10/sup 4/ transducing units per ml. Injection of 5- to 20-..mu..l volumes of vector directly beneath the blastoderm of unincubated chicken embryos led to infection of somatic stem cells. Infected embryos and adults contained unrearranged integrated proviral DNAs. Embryos expressed the transduced chicken growth hormone gene and contained high levels of serum growth hormone. Blood, brain, muscle, testis, and semen contained from individuals injected as embryos contained vector DNA. Replication-defective vectors of the reticuloendotheliosis virus transduced exogenous genes into chicken embryonic stem cells in vivo.

• Research Organization: Amgen Inc., Thousand Oaks, CA (USA)
• OSTI ID: 6017506
• Journal Information: J. Virol.; (United States), Vol. 63:6
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
CHICKENS
GENETIC ENGINEERING
DISEASE VECTORS
BIOLOGICAL FUNCTIONS
STH
GENES
DNA
EMBRYOS
GENE REGULATION
IN VIVO
STEM CELLS
VIRAL DISEASES
VIRUSES
ANIMAL CELLS
ANIMALS
BIRDS
DISEASES
FOWL
FUNCTIONS
HORMONES
INFECTIOUS DISEASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEPTIDE HORMONES
PITUITARY HORMONES
SOMATIC CELLS
VERTEBRATES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550900 - Pathology