Incorporation of dinitrophenyl-modified proteins within the 30S subunit of E. coli ribosomes
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6015774
Proteins were modified to varying extents (0.5 equivalent or more) with 2,4-(3,5-/sup 3/H)-dinitrofluorobenzene (DNFB) and separated from unmodified protein by RP-HPLC using dual wavelength monitoring at 214 and 360 nm. DNP-modified proteins were next reconstituted into 30S subunits as described by Held. Subunits were purified by sucrose gradient sedimentation and size exclusion-HPLC. The presence of DNP-modified protein was detected by radioactivity co-sedimenting with the 30S particle. RP-HPLC analysis of proteins extracted from 30S subunits containing modified proteins show a full complement of proteins.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 6015774
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Thesis/Dissertation
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Journal Article
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OSTI ID:5743147
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Journal Article
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Sun Sep 15 00:00:00 EDT 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5604651
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BENZENE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CELL CONSTITUENTS
CHROMATOGRAPHY
ESCHERICHIA COLI
FRACTIONATION
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PROTEINS
REAGENTS
RIBOSOMES
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BENZENE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CELL CONSTITUENTS
CHROMATOGRAPHY
ESCHERICHIA COLI
FRACTIONATION
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PROTEINS
REAGENTS
RIBOSOMES
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS