Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Contribution of vitamin K1 to the electron spin polarization in spinach photosystem I

Journal Article · · Biochemistry; (USA)
DOI:https://doi.org/10.1021/bi00487a006· OSTI ID:6014896
; ; ; ; ; ;  [1]
  1. Argonne National Laboratory, IL (USA)
+ Show Author Affiliations

The electron spin polarized (ESP) electron paramagnetic resonance (EPR) signal observed in spinach photosystem I (PSI) particles was examined in preparations depleted of vitamin K1 by solvent extraction and following biological reconstitution by the quinone. The ESP EPR signal was not detected in the solvent-extracted PSI sample but was restored upon reconstitution with either protonated or deuterated vitamin K1 under conditions that also restored electron transfer to the terminal PSI acceptors. Reconstitution using deuterated vitamin K1 resulted in a line narrowing of the ESP EPR signal, supporting the conclusion that the ESP EPR signals in the reconstituted samples arise from a radical pair consisting of the oxidized PSI primary donor, P700+, and reduced vitamin K1.

OSTI ID:
6014896
Journal Information:
Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:35; ISSN 0006-2960; ISSN BICHA
Country of Publication:
United States
Language:
English

Similar Records

Direct assignment of vitamin K sub 1 as the secondary acceptor A sub 1 in photosystem I
Journal Article · Thu Oct 31 23:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:5731840
EPR evidence that the photosystem I acceptor A/sub 1/ is a quinone molecule
Conference · Tue Dec 31 23:00:00 EST 1985 · OSTI ID:7150573
Rates of primary electron transfer reactions in the photosystem I reaction center reconstituted with different quinones as the secondary acceptor
Journal Article · Thu Oct 27 00:00:00 EDT 1994 · Journal of Physical Chemistry; (United States) · OSTI ID:6735630

Related Subjects

14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BIOLOGICAL FUNCTIONS
CHEMICAL REACTIONS
COAGULANTS
DEUTERIUM
DRUGS
ELECTRON SPIN RESONANCE
ELECTRON TRANSFER
EXTRACTION
FOOD
FUNCTIONS
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
QUINONES
REDOX REACTIONS
RESONANCE
SEPARATION PROCESSES
SOLVENT EXTRACTION
SPINACH
STABLE ISOTOPES
TRACER TECHNIQUES
VEGETABLES
VITAMIN K
VITAMINS