Polarity of fatty acid uptake and metabolism in a human intestinal cell line (CACO-2)

Conference · Sun Feb 25 23:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6011607

Trotter, P J; Storch, J ^[1]

---

1. Harvard School of Public Health, Boston, MA (United States)

Free fatty acids (ffa) can enter the intestinal cell via the apical (AP) or basolateral (BL) membrane. The authors are using the Caco-2 intestinal cell line to examine the polarity of ffa uptake and metabolism in the enterocyte. Cells are grown on permeable polycarbonate Transwell filters in order to obtain access to both AP and BL compartments. Differentiated Caco-2 cells form tight polarized monolayers which express small intestine-specific enzymes and are impermeable to the fluid phase marker Lucifer Yellow. Submicellar concentrations of {sup 3}H-palmitic acid (2uM) were added to AP or BL sides of Caco-2 monolayers at 37{degrees}C and cells were incubated for various times between 2 and 120 minutes. Total AP and BL uptake is similar; however, when relative membrane surface areas are accounted for, AP uptake is about 2-fold higher. The metabolism of AP and BL ffa is not significantly different: triacylglycerol and phosphatidylcholine account for most of the metabolites (32{plus minus}4 and 24{plus minus}2% respectively at 5 minutes). Little ffa oxidation is observed. Preincubation with albumin-bound 2-monoolein (100uM) and palmitate (50uM) increases the level of TG metabolites. The results suggest that in this cell line the uptake of AP ffa may be greater than BL ffa, but that AP (dietary) ffa and BL (plasma) ffa are metabolized similarly.

• OSTI ID: 6011607
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 4:3; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
DIET
DIGESTIVE SYSTEM
ENZYME ACTIVITY
GASTROINTESTINAL TRACT
HYDROGEN COMPOUNDS
INTESTINES
ISOTOPE APPLICATIONS
MATERIALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
SMALL INTESTINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE