Metabolism of medium- and long-chain fatty acids by isolated hepatocytes from small-for-gestational-age (SGA) and appropriate for-gestational-age (AGA) piglets

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6011583

Odle, J; Benevenga, N J; Crenshaw, T D ^[1]

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1. Univ. of Wisconsin, Madison (United States)

Hepatocytes were isolated from full-term, SGA and AGA piglets at 6 or 48 hours postpartum and were incubated with 1 mM (1-{sup 14}C)-octanoate (C8), -nonanoate (C9) or-oleate (C18:1). The cells oxidized (natom 1-C/(h 10{sup 6} cells)) C9 to Co{sub 2} (12.5) and acid soluble products (28.9) faster than C8 (10.9, 20.6, respectively), and both were oxidized faster than C18:1 (3.9, 9.9) regardless of the piglet age or weight. Oleate accumulated in lipid products 8-fold faster than C8 and C9. No differences between cells from SGA and AGA piglets were detected. Recovery of 1-C in CO{sub 2} was 48% higher in incubations with cells from 48 hours old than from 6 hour old piglets. This increase was attributable to a 70% higher oxygen consumption by 48 hour old cells. Theoretical oxygen consumption rates were computed from the fatty acid flux data and compared to measured oxygen consumption. hepatocytes from SGA and AGA piglets were equally capable of satisfying more that 57% of their energy needs from fatty acid oxidation. The oxygen consumption attributable to C9 metabolism was 30% higher than observed for C8 and C18:1. All fatty acids apparently spared endogenous fuels to a greater degree in 6 hour than in 48 hour piglets.

• OSTI ID: 6011583
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBOXYLIC ACIDS
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLISM
NEONATES
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
SOMATIC CELLS
SWINE
TRACER TECHNIQUES
VERTEBRATES