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Rapid extraction, radioiodination, and in vivo catabolism of 125I-labeled fibrinogen in the horse

Journal Article · · Am. J. Vet. Res.; (United States)
OSTI ID:6011328
Two methods were analyzed for the rapid extraction of equine fibrinogen from fresh plasma, using ammonium sulfate-sodium phosphate buffer. Fibrinogen from each of these 2 methods was then radiolabeled with 125I (half-life = 60.2 days, gamma = 35 keV), using monochloroiodine reagent. Mean protein-bound activity was 98.5% and mean clottable radioactivity was 94.1%. Radiolabeled fibrinogen administered IV to 15 horses had an overall mean (+/- SD) plasma half-life of 4.95 +/- 0.44 days.
Research Organization:
Univ. of California, Davis
OSTI ID:
6011328
Journal Information:
Am. J. Vet. Res.; (United States), Journal Name: Am. J. Vet. Res.; (United States) Vol. 12; ISSN AJVRA
Country of Publication:
United States
Language:
English

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