Partial purification of endogenous digitalis-like compound(s) in cord blood

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Clinical Chemistry (Winston-Salem, North Carolina); (USA)

OSTI ID:6011034

Balzan, S; Ghione, S; Biver, P; Gazzetti, P; Montali, U ^[1]

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1. C.N.R. Institute of Clinical Physiology, Pisa (Italy)

Increasing evidence indicates the presence of endogenous digitalis-like compound(s) in human body fluids. In this preliminary report, we describe a study of the partial purification by HPLC of these compounds in the plasma of neonates (who have particularly high concentrations of this substance) and adults. Plasma samples from neonates (cord blood) and adults, lyophilized and extracted with methanol, were applied on a 300 x 3.9 mm C18 Nova Pak column and eluted with a mobile phase of acetonitrile/methanol/water (17/17/66 or 14/14/72 by vol) and, after 30 min, with 100% methanol. We assayed eluted fractions for inhibitory activity of 86Rb uptake and for digoxin-like immunoreactivity. The elution profile revealed a first peak of inhibitory activity of 86Rb uptake at the beginning of the chromatography; another peak was eluted with the 100% methanol. The two peaks also cross-reacted with antidigoxin antibodies. Because the second peak could possibly reflect the nonspecific interference of various lipophilic compounds, we focused our attention on the first peak. For these fractions dose-response curves for 86Rb uptake and for displacement of digoxin were parallel, respectively, to those of ouabain and digoxin, suggesting similarities of digoxin-like immunoreactive substance to cardiac glycosides. Similar chromatographic profiles were also obtained for plasma from adults, suggesting that the endogenous glycoside-like compound(s) in the neonate may be the same as those in the adult.

• OSTI ID: 6011034
• Journal Information: Clinical Chemistry (Winston-Salem, North Carolina); (USA), Journal Name: Clinical Chemistry (Winston-Salem, North Carolina); (USA) Vol. 37:2; ISSN 0009-9147; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADULTS
AGE GROUPS
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHILDREN
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGITALIS
DIGITALIS GLYCOSIDES
DIGOXIN
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EXTRACTION
FRACTIONATION
GLYCOSIDES
IMMUNOASSAY
IMMUNOLOGY
INFANTS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEDICINAL PLANTS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
PLANTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOPES
SEPARATION PROCESSES
SOLVENT EXTRACTION
STROPHANTHINS
TRACER TECHNIQUES