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X-ray microprobe analysis of the retina and RPE in sheep with ovine ceroid-lipofuscinosis

Journal Article · · Neurobiology of Aging; (USA)
Ovine ceroid-lipofuscinosis (OCL) is one animal model for the human condition, and because autofluorescent lipopigments are prominent in the brain and eye, it may also prove useful as a model for aging. For example, a progressive decline in electrical recording from brain and retina are observed in both aging and OCL. Samples of retinal and retinal pigment epithelial (RPE) tissues were obtained from a young control. 2 animals with OCL and a normal aged sheep. Specimens were cryo-fractured and examined by scanning electron microscopy/x-ray microanalysis. Measurements made of 6 individual cells in the ganglion layer of OCL specimens, the remainder of the retina, and RPE showed age-related changes in zinc, iron, and copper which were associated with lipopigment accumulation in the RPE. There was marked decrease in phosphate, sulfur, and manganese levels, as photoreceptor cells and their outer segments are lost in the disease process. This is the first report of metal analysis in the retina and RPE in a disease entity, and as a function of normal aging.
OSTI ID:
6008445
Journal Information:
Neurobiology of Aging; (USA), Journal Name: Neurobiology of Aging; (USA) Vol. 11:6; ISSN 0197-4580; ISSN NEAGD
Country of Publication:
United States
Language:
English