In vitro synthesis of nitroxide free radicals by hog liver microsomes

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Free Radical Biology and Medicine; (USA)

DOI:https://doi.org/10.1016/0891-5849(90)90009-8· OSTI ID:6004692

Valvis, I I; Lischick, D; Shen, D; Sofer, S S ^[1]

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1. New Jersey Institute of Technology, Newark (USA)

The in vitro biooxidation of 4-hydroxy-2,2,6,6-tetra methylpiperidine (TEMP), 4-hydroxy-2,2,4,4-tetra methyl-1,3-oxazolidine (TEMO) and diphenylamine (DPA) by hog liver microsomes to their respective nitroxide free radicals, 4-hydroxy-2,2,6,6-tetra methylpiperidine-1-oxyl (TEMPO), 2,2,4,4-tetra methyl-1,3-oxazolidine-1-oxyl (TEMOO), and diphenylnitroxide (DPNO) has been investigated. For extending the life span of the liver microsomes, a calcium alginate immobilization procedure was used. The biooxidation rates of the above amines to their respective nitroxide metabolites were measured by means of oxygen uptake at 37 degrees C and pH 7.4. N-octylamine was found to be an activator in the biooxidation of the amines. The formation of the nitroxide radicals was identified by E.S.R. spectroscopy.

• OSTI ID: 6004692
• Journal Information: Free Radical Biology and Medicine; (USA), Journal Name: Free Radical Biology and Medicine; (USA) Vol. 9:4; ISSN FRBME; ISSN 0891-5849
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOSYNTHESIS
BODY
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELECTRON SPIN RESONANCE
ELEMENTS
GLANDS
IN VITRO
LIVER
MAGNETIC RESONANCE
MAMMALS
METABOLISM
MICROSOMES
NITROGEN COMPOUNDS
NITROGEN OXIDES
NONMETALS
ORGANOIDS
ORGANS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
RADICALS
REDOX REACTIONS
RESONANCE
RIBOSOMES
SWINE
SYNTHESIS
VERTEBRATES