Effect of low frequency low energy pulsing electromagnetic fields on mice injected with cyclophosphamide

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Experimental Hematology (Lawrence, Kansas); (USA)

OSTI ID:6003735

Cadossi, R; Zucchini, P; Emilia, G; Franceschi, C; Cossarizza, A; Santantonio, M; Mandolini, G; Torelli, G ^[1]

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1. Univ. of Modena (Italy)

C3H mice have been used to investigate the effect of a combination of cyclophosphamide (CY) and electromagnetic fields (PEMF). Mice were injected i.p. with a single dose of 200 mg/kg body weight of CY and then exposed to PEMF 24 h per day. In an initial series of experiments immediately after CY injection mice were exposed to PEMF until sacrifice. WBC counts in the peripheral blood demonstrated a quicker decline in WBC at days 1 and 2 in mice exposed to PEMF. Groups of mice were sacrificed at days 1, 4, 6, 8, and 10 after CY injection. In mice exposed to PEMF the spleen weight was less than in controls at days 6, 8, and 10. Autoradiographic studies demonstrated that the labeling index of bone marrow smears did not significantly differ between controls and experimental mice exposed to PEMF, whereas the spleen labeling index proved to be higher among control mice versus mice exposed to PEMF at day 6, and higher among mice exposed to PEMF versus controls at day 8. In a second series of experiments mice were exposed to PEMF only over the 24 h following CY injection. We found that the spleens of mice exposed to PEMF weighed less than those of controls at days 6 and 8. The labeling index of bone marrow did evidence a slight decrease among mice exposed to PEMF at days 8 and 10 after CY injection versus control mice. The spleen labeling index proved to be lower in experimental mice exposed to PEMF than in controls at days 4, 6, and 8. Mice were then injected with CY, half were exposed to PEMF, and 24 h later bone marrow was recovered from both groups of animals. The same number of bone marrow cells was injected via the tail vein into recipient mice irradiated to 8.5 Gy.

• OSTI ID: 6003735
• Journal Information: Experimental Hematology (Lawrence, Kansas); (USA), Journal Name: Experimental Hematology (Lawrence, Kansas); (USA) Vol. 19:3; ISSN EXHMA; ISSN 0301-472X
• Country of Publication: United States
• Language: English

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560400* -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BLOOD COUNT
BODY
BONE MARROW CELLS
CELL DIVISION
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
IMMUNOSUPPRESSIVE DRUGS
MAMMALS
MICE
ORGANS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN
SYNERGISM
VERTEBRATES