Characterization of fatty alcohol:NAD/sup +/ oxidoreductase from rat liver
Long chain fatty alcohols are known to be rapidly oxidized to the corresponding fatty acids in normal mammalian tissues in vivo. However, the enzyme system responsible for the reaction has not been well documented. We have demonstrated the conversion of (1-/sup 14/C)hexadecanol to (1-/sup 14/C)hexadecanoic acid using a cell-free system from rat liver. The subcellular distribution of this enzyme system parallels that of microsomal marker enzymes. The enzyme showed an absolute requirement for NAD/sup +/ and had a pH optimum of 8.4. Neither NADP/sup +/, NADH, nor NADPH can substitute for NAD/sup +/ as a cofactor. The reaction does not require oxygen. Albumin increases the rate of the reaction. The enzymic activity is not affected by including pyrazole, sodium azide, or AMP, ATP, and quinacrine in the incubation mixture. The apparent K/sub m/ for hexadecanol is 0.67 ..mu..M and the V/sub max/ is 5.98 nmol/min/mg of protein. The reaction product was identified as the fatty acid by both thin-layer and gas-liquid chromatography. Fatty aldehyde was also isolated and identified from the reaction mixture. Addition of semicarbazide to the incubation system increased the amount of fatty aldehyde produced with concomitant decrease in the formation of fatty acid. This documents that aldehyde is an intermediate in the reaction. This oxidoreductase is found in most tissues but has the highest activity in liver.
- Research Organization:
- Oak Ridge Associated Univ., TN
- DOE Contract Number:
- EY-76-C-05-0033
- OSTI ID:
- 6001925
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:8; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ALCOHOLS
ALDEHYDES
AMP
ANIMALS
ATP
AZIDES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BODY
CARBON 14 COMPOUNDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COENZYMES
DATA
DATA FORMS
DECANOLS
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
GLANDS
HETEROCYCLIC COMPOUNDS
HEXADECANOIC ACID
HYDROXY COMPOUNDS
IN VITRO
INFORMATION
ISOLATED VALUES
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
MONOCARBOXYLIC ACIDS
NAD
NITROGEN COMPOUNDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDATION
OXIDOREDUCTASES
PROTEINS
PYRAZOLES
RATS
REACTION KINETICS
RODENTS
SEMICARBAZIDES
TISSUE EXTRACTS
TISSUES
VERTEBRATES