Alterations of neutral glycolipids in cells infected with syncytium-producing mutants of herpes simplex virus type 1

Journal Article · Tue Nov 01 00:00:00 EST 1977 · J. Virol.; (United States)

OSTI ID:6000935

Ruhlig, M A; Person, S

The isolation of syncytium-producing mutants of herpes simplex virus type 1 (KOS strain), which cause extensive cell fusion during otherwise normal infections, has been reported previously (S. Person, R.W. Knowles, G.S. Read, S.C. Warner, and V.C. Bond, J. Virol. 17:183-190, 1976). Seven of these mutants, plus two syncytial strains obtained elsewhere were used to compare the incorporation of labeled galactose into neutral glycolipids of mock-infected, wild-type-infected, and syncytially infected human embryonic lung cells. Five predominant cellular glycolipid species were observed, denoted GL-1 through GL-5 in order of increasing oligosaccharide chain length; for example, GL-1 and GL-2 correspond to glycolipids that contain mono- and disaccharide units, respectively. Wild-type virus infection caused an increase in galactose incorporation into GL-1 and GL-2 relative to GL-3 through GL-5. For a single labeling interval from 4 to 10 h after adsorption, syncytial infections generally resulted in a relatively greater incorporation into more complex glycolipids than did wild-type infections. One mutant, syn 20, was compared with wild-type virus throughout infection by using a series of shorter labeling pulses and appeared to delay by at least 2 h the alterations observed during wild-type infections. These alterations are apparently due to defects in synthesis, since prelabeled cellular glycolipids were not differentially degraded during mock or virus infection.

• Research Organization: Pennsylvania State Univ., University Park
• OSTI ID: 6000935
• Journal Information: J. Virol.; (United States), Vol. 24:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HERPES SIMPLEX
MUTANTS
INFECTIOUS DISEASES
GLYCOLIPIDS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
GALACTOSE
LABELLING
OLIGOSACCHARIDES
VIRUSES
ALDEHYDES
CARBOHYDRATES
CHEMISTRY
DISEASES
HEXOSES
LIPIDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
SACCHARIDES
SKIN DISEASES
SYNTHESIS
550901* - Pathology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques