Active inhibition of herpes simplex virus type 1-induced cell fusion
Previous studies have demonstrated that syn mutant-infected cells fuse less well with nonsyncytial virus-infected cells than with uninfected cells, a phenomenon defined as function inhibition. The present study characterizes the kinetics as well as the requirements for expression of fusion inhibition. Initially, the capacity of sparse syn mutant-infected cells to fuse with uninfected surrounding cells was determined throughout infection. Of seven syn mutants examined, including representatives with alterations in two different viral genes that affect cell fusion, all showed an increase in fusion capacity up to 12 hr after infection and a decrease at later times. Fusion inhibition was examined in experiments employing sparse syn20-infected cells which had been incubated to a maximum fusion capacity; it was shown that surrounding cells infected with KOS, the parent of syn20, began to inhibit fusion by the syn20-infected cells at about 4 hr after infection, and that the maximum ability to inhibit fusion was attained at about 6 hr after infection. The metabolic blocking agents actinomycin D (RNA), cycloheximide (protein), 2-deoxyglucose, and tunicamycin (glycoslyation of glycoproteins) all showed the ability to inhibit the expression of fusion inhibition by KOS-infected cells if added shortly after infection. It is concluded that fusion inhibition is an active process that requires the synthesis of RNA, proteins, and glycoproteins. 17 references, 3 figures, 2 tables.
- Research Organization:
- Pennsylvania State Univ., University Park
- OSTI ID:
- 6000665
- Journal Information:
- Virology; (United States), Vol. 117
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HERPES SIMPLEX
INHIBITION
HYBRIDOMAS
INFECTIVITY
ACTINOMYCIN
BIOCHEMICAL REACTION KINETICS
CYCLOHEXIMIDE
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
GENES
METABOLISM
MUTANTS
PROTEINS
RNA
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
DATA
DISEASES
DRUGS
FUNGICIDES
INFECTIOUS DISEASES
INFORMATION
KINETICS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PESTICIDES
REACTION KINETICS
SKIN DISEASES
VIRAL DISEASES
550700* - Microbiology