Evaluation of electrical aggregometry: comparison with optical aggregometry, secretion of ATP, and accumulation of radiolabeled platelets

Journal Article · Sat Jan 01 04:00:00 EST 1983 · J. Lab. Clin. Med.; (United States)

OSTI ID:5998844

Ingerman-Wojenski, C; Smith, J B; Silver, M J

Platelet aggregation has been most commonly studied in vitro by measuring increases in light transmission as platelets aggregate in PRP (platelet-rich plasma). Recently, an electrical impedance method for measuring platelet aggregation has been introduced. This method can be used with either PRP or whole blood and measures an increase in impedance across electrodes placed in the blood samples as platelets accumulate on them. Results obtained by the two methods were compared using ADP and collagen as aggregating agents, and also have measured the secretion of platelet ATP simultaneously. Although the aggregometry results were similar, recordings obtained by the electrical method did not distinguish two waves of platelet aggregation or correlate with secretion as well as recordings obtained by the optical method. When PGI/sub 2/ (prostacyclin) or PGE/sub 1/ (prostaglandin E/sub 1/) was added to the PRP, both the rate and extent of the increase in light transmittance were inhibited, but the main effect on the increase in impedance was a decrease in its rate and not in its extent. Increases in impedance and secretion of ATP were also measured in whole blood after the platelets had been labeled with a /sup 125/I-containing antibody specific for platelet surface glycoproteins. It appeared that the increases in impedance lagged several minutes behind the formation of platelet aggregates and the secretion of platelet ATP.

• Research Organization: Thomas Jefferson Univ., Philadelphia, PA
• OSTI ID: 5998844
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 101:1; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
AGGLOMERATION
ANTIBODIES
ATP
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRIC IMPEDANCE
ELECTRON CAPTURE RADIOISOTOPES
HALOGEN COMPOUNDS
IMPEDANCE
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
MATERIALS
MEASURING METHODS
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
RADIOISOTOPES
SECRETION
TRACER TECHNIQUES