Extraction procedure for the determination of trace chromium in plasma by proton-induced x-ray emission spectrometry

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00267a036· OSTI ID:5998580

Simonoff, M; Llabador, Y; Hamon, C; Simonoff, G N

A method is described for the determination of Cr/sup 3 +/ in blood serum by proton-induced X-ray emission (PIXE) after wet ashing and an extractive enrichment procedure. Wet ashing of 5 to 10 mL of human plasma is carried out with nitric, perchloric, and sulfuric acids. Chromium(VI) is complexed with ammonium pyrrolidinecarbodithioate-methyl isobutyl ketone (APCD-MIBK) at a pH of 2.4. After evaporation of the organic phase the metallic residue is dissolved in nitric and hyrochloric acids. A known volume of a 50 mg/L nickel solution is added as an internal standard and the solution reconcentrated. The few residual microliters are deposited on a polycarbonate foil. Recovery for this preconcentration step is measured with the use of carrier-free /sup 51/Cr. Satisfactory values are found for chromium in three NBS Standard Reference Materials. The detection limit of this analytical procedure is about 0.3 ng/mL. 9 references, 3 figures, 1 table.

• Research Organization: Centre d'Etudes Nucleaires de Bordeaux-Gradignan, France
• OSTI ID: 5998580
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 56:3; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400103* -- Radiometric & Radiochemical Procedures-- (-1987)
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BLOOD SERUM
BODY FLUIDS
CHEMICAL ANALYSIS
CHROMIUM
DATA
ELEMENTS
EXPERIMENTAL DATA
INFORMATION
MATERIALS
METALS
NONDESTRUCTIVE ANALYSIS
NUMERICAL DATA
PH VALUE
PIXE ANALYSIS
QUANTITATIVE CHEMICAL ANALYSIS
SPECTRA
TRANSITION ELEMENTS
X-RAY EMISSION ANALYSIS
X-RAY SPECTRA