Localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein D that stimulates the production of neutralizing antibody

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Virol.; (United States)

OSTI ID:5997869

Cohen, G H; Dietzschold, B; Ponce de Leon, M; Long, D; Golub, E; Varrichio, A; Pereira, L; Eisenberg, R J

An antigenic determinant capable of inducing type-common herpes simplex virus (HSV)-neutralizing antibodies has been located on glycoprotein D (gD) of HSV type 1 (HSV-1). A peptide of 16 amino acids corresponding to residues 8 to 23 of the mature glycoprotein (residues 33 to 48 of the predicted gD-1 sequence) was synthesized. This peptide reacted with an anti-gD monoclonal antibody (group VII) previously shown to neutralize the infectivity of HSV-1 and HSV-2. The peptide was also recognized by polyclonal antibodies prepared against purified gD-1 but was less reactive with anti-gD2 sera. Sera from animals immunized with the synthetic peptide reacted with native gD and neutralized both HSV-1 and HSV-2.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5997869
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 49:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIBODY FORMATION
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
GLUCOPROTEINS
HERPES SIMPLEX
IMMUNE SERUMS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MICE
MICROORGANISMS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PROTEINS
RABBITS
RADIOASSAY
RADIOISOTOPES
RODENTS
SACCHARIDES
SKIN DISEASES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
VIRAL DISEASES
VIRUSES