Monoclonal antibody to the gastrin receptor on parietal cells recognizes a 78-kDa protein

Journal Article · Fri May 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.9.2698· OSTI ID:5997779

Mu, F T; Baldwin, G; Weinstock, J; Stockman, D; Toh, B H

Four monoclonal antibodies reactive by immunofluorescence and by flow microfluorimetry with canine and porcine gastric parietal cell membranes were produced by fusion of mouse NS-1 myeloma cells with splenocytes from mice immunized with a population of canine gastric mucosal cells containing 60-70% parietal cells. One of these, an IgM antibody designated 2Cl, reacted with the surface membranes of parietal cells by immunofluorescence, flow microfluorimetry, and immunogold electron microscopy; competed with /sup 125/I-labeled gastrin for binding to gastric cells; and inhibited by 56% maximal gastrin stimulation of (/sup 14/C)aminopyrine uptake in parietal cells. The antibody immunoprecipitated /sup 125/I-labeled samples of a 78-kDa gastrin-binding protein purified from membrane extracts of porcine gastrin mucosa but did not recognize the same protein labeled covalently with /sup 125/I-labeled gastrin-(2-17)-hexadecapeptide. These observations suggest that the previously identified 78-kDa gastrin-binding protein is the gastrin receptor and that the antibody 2C1 is directed against the gastrin binding site of the gastrin receptor.

• Research Organization: Monash Univ. Medical School, Prahran, Australia
• OSTI ID: 5997779
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:9; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CONFIGURATION INTERACTION
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
GASTRIN
GASTROINTESTINAL TRACT
HORMONES
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
MEMBRANE PROTEINS
MEMBRANES
MICROSCOPY
MONOCLONAL ANTIBODIES
MUCOUS MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PRECIPITATION
PROTEINS
RADIOISOTOPES
RECEPTORS
SEPARATION PROCESSES
STOMACH
TRANSMISSION ELECTRON MICROSCOPY