Effect of endotoxin-induced monokines on glucose metabolism in the muscle cell line L6

Journal Article · Fri May 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.9.2590· OSTI ID:5997730

Lee, M D; Zentella, A; Pekala, P H; Cerami, A

Exposure of fully differentiated L6 myotubes to a crude monokine preparation from endotoxin-stimulated RAW 264.7 cells resulted in a rapid and substantial (70%) increase in fructose 2,6-bisphosphate concentration coincident with a depletion of cellular glycogen and an increased lactate production. During the time required for glycogen depletion (3 hr), stimulation of 3-O-methyl-D-glucose and 2-deoxy-D-glucose uptake was initiated and observed to reach a maximum enhancement of 200% 12-15 hr later. The monokine had no effect on the K/sub m/ value for 2-deoxy-D-glucose uptake (1.1 mM), while V/sub max/ was increased from 912 to 2400 pmol/min per mg of protein. The increase was cytochalasin B inhibitable and was dependent on protein synthesis. Photoaffinity labeling and equilibrium binding studies with (/sup 3/H)cytochalasin B support the hypothesis that this increase in hexose transport was due to an increase in hexose transporters present in the plasma membrane. Purified recombinant interleukin-1..cap alpha.. had no effect on hexose transport, whereas purified recombinant cachectin/tumor necrosis factor did stimulate hexose uptake, with half-maximal stimulation occurring at 36 nM. Although cachectin accounts for most of the biological activity associated with the crude monokine preparation, it is not the only monokine capable of inducing glucose transport in L6 cells. Specific immunoabsorption of cachectin/tumor necrosis factor from the crude monokine preparation revealed a monokine that had a similar bioactivity at extremely low concentrations on L6 cells.

• Research Organization: East Carolina Univ., Greenville, NC
• OSTI ID: 5997730
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:9; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
FIBROBLASTS
GLUCOSE
GLYCOGEN
HEXOSES
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MEMBRANES
METABOLISM
MICE
MICROSOMES
MONOCYTES
MONOSACCHARIDES
MUSCLES
ORGANIC COMPOUNDS
ORGANOIDS
PHAGOCYTES
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
TRITIUM COMPOUNDS
VERTEBRATES