Light regulation and localization of OEC-33 in maize

Conference · Fri Aug 01 04:00:00 EDT 1986

OSTI ID:5997152

Sutton, A; Sieburth, L; Bennett, J

The 33 kDa extrinsic protein of PSII (OEC-33) is synthesized in the cytoplasm as a 39 kDa precursor is transported across the chloroplast envelope and thylakoid membrane and becomes attached to the inner surface of the thylakoid membrane. This protein, along with several other extrinsic and intrinsic polypeptides, forms the oxygen evolving complex of PSII; when the extrinsic proteins are removed from PSII particles by washing with alkaline Tris, oxygen evolution is abolished. Because OEC-33 is a component of PSII, we were interested in determining how closely the accumulation of this protein is coordinated with that of the intrinsic PSII proteins. We have isolated polyspecific antibodies against OEC-33 and have used the antiserum to determine the role of phytochrome and chlorophyll biosynthesis in the accumulation of this polypeptide in maize. During the course of these studies we have obtained information about the localization of OEC-33 in plants grown under conditions where several other components of PSII fail to accumulate. 12 refs., 5 figs.

• Research Organization: Brookhaven National Lab., Upton, NY (USA); Georgia Univ., Athens (USA). Dept. of Botany
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 5997152
• Report Number(s): BNL-39083; CONF-860808-13; ON: DE87011290
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CEREALS
CHEMICAL REACTIONS
ELECTROPHORESIS
ENZYME INDUCTION
GENE REGULATION
GRASS
MAIZE
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYTOCHROMES
PIGMENTS
PLANTS
PROTEINS
SYNTHESIS
THYLAKOID MEMBRANE PROTEINS