Stimulation of proliferation, differentiation, and function of human cells by primate interleukin 3
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Cloned gibbon interleukin 3 (gIL-3) was found to stimulate the proliferation and differentiation of human bone marrow cells to produce day-14 granulocyte, macrophage, granulocyte-macrophage, and eosinophil colonies in semisolid agar. In the presence of normal human plasma, gIL-3 stimulated megakaryocytes. In methylcellulose cultures, it stimulated erythroid colonies in the presence, but not in the absence, of erythropoietin. When mature human leukocytes were used, gIL-3 stimulated the function of purified mature eosinophils as measured by the capacity to kill /sup 51/Cr-labeled antibody-coated target cells, to produce superoxide anions, and to phagocytize opsonized yeast particles in a manner similar to recombinant human granulocyte-macrophage colony-stimulating factor. In contrast, gIL-3 did not significantly stimulate any of the neutrophil functions tested, whereas human recombinant granulocyte-macrophage colony-stimulating factor was active in these assay. Among cytokines that are active on human hematopoietic cells, gIL-3 thus has a distinct set of functions and may predict the range of actions of the human molecule.
- Research Organization:
- Institute of Medical and Veterinary Science, Adelaide, Australia
- OSTI ID:
- 5997018
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:9; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CELL PROLIFERATION
CHEMISTRY
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOKINES
MACROPHAGES
MATERIALS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CELL PROLIFERATION
CHEMISTRY
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOKINES
MACROPHAGES
MATERIALS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES