Selection and characterization of a glucokinase-deficient mutant of Tetrahymena thermophila

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.4.378· OSTI ID:5996287

Roberts, Jr, C T; Lavine, J E; Morse, D E

The authors isolated a mutant of Tetrahymena thermophila that is resistant to inhibition of growth by the glucose analog 2-deoxyglucose. The mutant exhibits a deficiency in a cytoplasmic glucokinase. This enzymatic defect and the attendant inability to convert 2-deoxyglucose to toxic phosphorylated derivatives is apparently the sole basis for the mutant phenotype since transport of glucose and 2-deoxyglucose is unimpaired; there is no elevation of glucose-6-phosphatase activity, which could decrease the level of toxic 2-deoxyglucose metabolites. Genetic analyses have shown that the mutant allele is recessive and inherited as a single Mendelian mutation. The glucokinase-deficient strain described here is useful for the selection of other mutants in this organism and for the investigation of various cellular processes initiated or modulated by glucose and its analogs. The authors have exploited the molecular defect in this strain to investigate the initial steps in the cyclic AMP-mediated repression of galactokinase gene expression which is caused by glucose.

• Research Organization: Dept. of Biological Sciences and the Marine Science Institute, Univ. of California, Santa Barbara, CA 93106
• OSTI ID: 5996287
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:4; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMP
ANIMALS
ANTIMETABOLITES
BIOLOGICAL ADAPTATION
BIOLOGY
BIOSYNTHESIS
CARBOHYDRATES
CELL CONSTITUENTS
CILIATA
CYTOPLASM
DISEASES
DRUGS
ENZYME INDUCTION
ENZYMES
ESTERASES
GALACTOSE
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
GENETICS
GLUCOSE
GROWTH
HEXOSES
HYDROLASES
INHIBITION
INVERTEBRATES
MAPPING
METABOLIC DISEASES
METABOLISM
METABOLITES
MICROORGANISMS
MONOSACCHARIDES
MUTANTS
MUTATIONS
NUCLEOPROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHENOTYPE
PHOSPHATASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
PROTOZOA
SACCHARIDES
SIMULATION
SYNTHESIS
TEMPERATURE EFFECTS
TETRAHYMENA
TOXICITY
TRANSFERASES