Free-living dinitrogen-fixing bacteria isolated from petroleum refinery oily sludge

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5990983

Laguerre, G; Bossand, B; Bardin, R

Dinitrogen-fixing activity (acetylene reduction and /sup 15/N/sub 2/ fixation) was found in an oily sludge originating from a petroleum refinery. Two representative dinitrogen-fixing bacterial strains were isolated from this oily waste. Their nitrogenase activity was effective when they were cultivated on sterilized sludge or simple carbon substrates (organic acid salts, sugars). Using the classical methods, these strains could not be unambiguously related to other diazotrophic taxa. The landfarming process is widely used for oily sludge disposal; this study shows that oily sludges are more than a simple carbon input into the soil but that they must also be considered as real sources of dinitrogen-fixing and probably degradative microorganisms.

• Research Organization: Universite Claude Bernard, Villeurbanne, France
• OSTI ID: 5990983
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:7; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
020900* -- Petroleum-- Environmental Aspects
510200 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550501 -- Metabolism-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
ENERGY SOURCES
ENZYME ACTIVITY
ENZYMES
FOSSIL FUELS
FUELS
GROUND DISPOSAL
GROWTH
INDUSTRIAL PLANTS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MANAGEMENT
MICROORGANISMS
NITRO-GROUP DEHYDROGENASES
NITROGEN 15
NITROGEN FIXATION
NITROGEN ISOTOPES
NITROGENASE
NUCLEI
ODD-EVEN NUCLEI
OXIDOREDUCTASES
PETROLEUM
PETROLEUM FRACTIONS
PETROLEUM REFINERIES
PETROLEUM RESIDUES
SOILS
STABLE ISOTOPES
TRACER TECHNIQUES
WASTE DISPOSAL
WASTE MANAGEMENT