Effects of adrenalectomy on the control and adrenergic regulation of cytosolic free calcium in hepatocytes
The purpose of this study was to investigate the effects of adrenalectomy on the control and ..cap alpha..-adrenergic regulation of the concentration of cytosolic free calcium (Ca/sub i/) in hepatocytes. In hepatocytes isolated from adrenalectomized (adx) and sham-operated male rats 7-1 days after surgery, Ca/sub i/ at rest and in response to epinephrine (EPI) was measured with the calcium-sensitive photoprotein aequorin, /sup 45/Ca efflux was measured, and Ca/sup 2 +/ release from intracellular stores in response to inositol triphosphate (IP/sub 3/) was measured in saponin-permeabilized cells. Liver calmodulin content was also assayed by radioimmunoassay. It was found in adx rats that the resting Ca/sub i/ was elevated, the rise in Ca/sub i/ during EPI stimulation was reduced at physiological EPI concentrations, and the rise in calcium efflux evoked by EPI was reduced. Furthermore, the slope of the relationship between Ca/sub i/ and calcium efflux was reduced 60% in adx. Adx did not alter the characteristics of Ca/sup 2 +/ release from intracellular calcium pools in response to IP/sub 3/ in permeabilized cells. Finally, the liver calmodulin contents were not significantly different between the 2 groups.
- Research Organization:
- Pittsburgh Univ., PA (USA)
- OSTI ID:
- 5990512
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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ADRENALINE
BIOCHEMICAL REACTION KINETICS
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
CALCIUM 45
CALMODULIN
CELL MEMBRANES
LIVER CELLS
PERMEABILITY
RADIOIMMUNOASSAY
RATS
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ALKALINE EARTH ISOTOPES
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ANIMAL CELLS
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INTERMEDIATE MASS NUCLEI
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550201* - Biochemistry- Tracer Techniques