Presentation of human minor histocompatibility antigens by HLA-B35 and HLA-B38 molecules
- Univ. of Tokyo (Japan)
Cytotoxic T lymphocyte (CTL) clones specific for human minor histocompatibility antigens (hmHAs) were produced from a patient who had been grafted with the kidneys from his mother and two HLA-identical sisters. Of eight CTL clones generated, four recognized an hmHA (hmHA-1) expressed on cells from the mother and sister 3 (second donor); two recognized another antigen (hmHA-2) on cells from the father, sister (third donor), and sister 3; and the remaining two clones recognized still another antigen (hmHA-3) on cells from the father and sister 3. Panel studies revealed that CTL recognition of hmHA-1 was restricted by HLA-B35 and that of hmHA-2 and hmHA-3 was restricted by HLA-B38. The HLA-B35 restriction of the hmHA-1 -specific CTL clones was substantiated by the fact that they killed HLA-A null/HLA-B null Hmy2CIR targets transfected with HLA-B35 but not HLA-B51, -Bw52, or -Bw53 transfected Hmy2CIR targets. These data demonstrated that the five amino acids substitutions on the {alpha}{sub 1} domain between HLA-B35 and -Bw53, which are associated with Bw4/Bw6 epitopes, play a critical role in the relationship of hmHA-1 to HLA-B35 molecules. The fact that the hmHA-1-specific CTLs failed to kill Hmy2CIR cells expressing HLA-B35/51 chimeric molecules composed of the {alpha}{sub 1} domain of HLA-B35 and other domains of HLA-B51 indicated that eight residues on the {alpha}{sub 2} domain also affect the interaction of hmHA-1 and the HLA-B35 molecules.
- OSTI ID:
- 5989541
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:7; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINO ACID SEQUENCE
ANIMAL CELLS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL KILLING
CHROMATES
CHROMIUM 51
CHROMIUM COMPOUNDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GRAFT-HOST REACTION
HISTOCOMPATIBILITY COMPLEX
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
ISOTOPES
KIDNEYS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
ORGANS
OXYGEN COMPOUNDS
PATIENTS
RADIOISOTOPES
SECRETION
SODIUM COMPOUNDS
SOMATIC CELLS
TRANSITION ELEMENT COMPOUNDS
TRANSPLANTS