Diacylglycerol production induced by growth hormone in Ob1771 preadipocytes arises from phosphatidylcholine breakdown
- Universite de Nice-Sophia Antipolis (France)
Growth Hormone has recently been shown to stimulate the formation of diacylglycerol in Ob1771 mouse preadipocyte cells without increasing inositol lipid turnover. Addition of growth hormone to Ob1771 cells prelabelled with ({sup 3}H)glycerol or ({sup 3}H)choline led to a rapid, transient and stoechiometric formation of labelled diacylglycerol and phosphocholine, respectively. In contrast, no change was observed in the level of choline and phosphatidic acid whereas the release of water-soluble metabolites in ({sup 3}H)ethanolamine prelabelled cells exposed to growth hormone was hardly detectable. Stimulation by growth hormone of cells prelabelled with (2-palmitoyl 9, 10 ({sup 3}H))phosphatidylcholine also induced the production of labelled diacyglycerol. Pertussis toxin abolished both diacylglycerol and phosphocholine formation induced by growth hormone. It is concluded that growth hormone mediates diacylglycerol production in Ob1771 cells by means of phosphatidylcholine breakdown involving a phospholipase C which is likely coupled to the growth hormone receptor via a pertussis toxin-sensitive G-protein.
- OSTI ID:
- 5989455
- Journal Information:
- Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 173:3; ISSN BBRCA; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIGENS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOXYLESTERASES
CHOLINE
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DRUGS
ENZYMES
ESTERASES
ESTERS
FAT CELLS
GLYCEROL
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPASES
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METABOLISM
MICE
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY HORMONES
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
STH
SYNTHESIS
TISSUES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES