Astrocyte cultures derived from human brain tissue express angiotensinogen mRNA

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5720· OSTI ID:5988512

Milsted, A; Barna, B P; Ransohoff, R M; Brosnihan, K B; Ferrario, C M ^[1]

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1. Cleveland Clinic Foundation, OH (USA)

The authors have identified human cultured cell lines that are useful for studying angiotensinogen gene expression and its regulation in the central nervous system. A model cell system of human central nervous system origin expressing angiotensinogen has not previously been available. Expression of angiotensinogen mRNA appears to be a basal property of noninduced human astrocytes, since astrocytic cell lines derived from human glioblastomas or nonneoplastic human brain tissue invariably produced angiotensinogen mRNA. In situ hybridization histochemistry revealed that angiotensinogen mRNA production was not limited to a subpopulation of astrocytes because >99% of cells in these cultures contained angiotensinogen mRNA. These cell lines will be useful in studies of the molecular mechanisms controlling angiotensin synthesis and the role of biologically active angiotensin in the human brain by allowing the authors to examine regulation of expression of the renin-angiotensin system in human astrocyte cultures.

• OSTI ID: 5988512
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:15; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
BRAIN
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DRUGS
ENZYMES
FIBROBLASTS
GENE REGULATION
GLOBULINS
HYBRIDIZATION
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
NERVOUS SYSTEM
NONSPECIFIC PEPTIDASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RENIN
RNA
SOMATIC CELLS
TUMOR CELLS
VASOCONSTRICTORS
VERTEBRATES