Linkage of two human pregnancy-specific. beta. sub 1 -glycoprotein genes: One is associated with hydatidiform mole

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5822· OSTI ID:5988108

Leslie, K K; Watanabe, Shuichiro; Lei, Kejian; Chou, D Y; Plouzek, C A; Deng, Hweichuang; Torres, J; Chou, J Y ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

A genomic clone containing two linked human pregnancy-specific {beta}{sub 1}-glycoprotein (PS{beta}G) genes has been isolated and characterized. The two genes are arranged in the same 5{prime} {yields} 3{prime} orientation; the 3{prime} region (including the A2 and B-C exons) of the upstream gene, PSGGA, is linked to the 5{prime} region (including the 5{prime}/L and L/N exons) of PSGGB, the downstream gene. Depending upon the domains compared, PSGGA and PSGGB share 92-98% nucleotide and 86-95% amino acid sequence identity with PSG93, the most abundant PS{beta}G transcript. Northern blot hybridization performed with a PSGGB-specific oligonucleotide probe to the N domain revealed that PSGGB or a PSGGB-like gene encodes a major 1.7-kilobase mRNA in hydatidiform mole tissues and a major 2.0-kilobase mRNA in term placenta tissues. Moreover, the PSGGB-specific probe hybridized most strongly with mRNA from molar trophoblastic tissue, suggesting that the PSGGB-like species may be the gene preferentially expressed in gestational trophoblastic disease. Additionally, the sequence of a 2,315-base-pair PS{beta}G cDNA (PSG95) that contains an N-A1-A2-B2-C domain arrangement is reported. The coding region of PSG95 is identical to the previously reported cDNA clones PSG1d and FL-NCA, but PSG95 contains an additional 518 and 523 base pairs in the 3{prime} end as compared with PSG1d and FL-NCA, respectively.

• OSTI ID: 5988108
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
GENETIC MAPPING
MESSENGER-RNA
DNA HYBRIDIZATION
PREGNANCY
BIOCHEMISTRY
RECOMBINANT DNA
DNA SEQUENCING
AMINO ACID SEQUENCE
GENES
MAN
PLACENTA
ANIMALS
CHEMISTRY
DNA
FETAL MEMBRANES
HYBRIDIZATION
MAMMALS
MAPPING
MEMBRANES
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550401* - Genetics- Tracer Techniques