Mating system and basidiospore formation in the lignin-degrading basidiomycete Phanerochaete chrysosporium
Prototrophic strains recovered from crosses between auxotrophic strains of the lignin-degrading basidiomycete Phanerochaete chrysosporium were induced to fruit. The progeny of most of these self-crosses were prototrophic, indicating that the nuclei of the original prototroph were wild-type recombinants rather than complementary heterokaryons and that the binucleate basidiospores of this organism are homokaryotic. Various wild-type strains were shown to have multinucleate cells lacking clamp connections and to possess a variable number of sterigmata per basidium. Colonies arising from single conidia of various wild-type strains were all capable of producing fruit bodies and basidiospores. In addition, single basidiospores from three wild-type strains all produced fruit bodies and basidiospores. Nonfruiting as well as fruiting isolates were obtained from single basidiospores of five other wild-type strains. Basidiospores from these fruiting isolates always yielded colonies that fruited, again indicating that the spores are homokaryotic. Nonfruiting isolates from the same strain did not produce basidiospores when allowed to form a heterokaryon, implying that these isolates do not represent mating types. All this evidence indicates that P. chrysosporium has a primary homothallic mating system. In addition to fruiting and nonfruiting phenotypes, basidiospores from strain OGC101, a derivative of ME-446, gave rise to colonies which did not grow on cellulose (Cel/sup -/). The fruiting, nonfruiting, and Cel/sup -/ phenotypes differed from each other and from the parental wild-type strain in a variety of characteristics, including growth, conidiation, and evolution of /sup 14/CO/sub 2/ from /sup 14/C-side chain-labeled lignin, indicating that strain OCG101 is a heterokaryon.
- Research Organization:
- Oregon Graduate Center, Beaverton
- DOE Contract Number:
- FG06-86ER13550
- OSTI ID:
- 5987255
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 53:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LIGNIN
BIODEGRADATION
PHANEROCHAETE
REPRODUCTION
CARBON 14 COMPOUNDS
CARBON DIOXIDE
CELL NUCLEI
CONIDIA
PHENOTYPE
PROGENY
SPORES
TRACER TECHNIQUES
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
DECOMPOSITION
FUNGI
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550701 - Microbiology- Tracer Techniques